• Immunofluorescence analysis of U2OS cells using RPH3AL Rabbit polyclonal antibody (STJ26615) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using RPH3AL Rabbit polyclonal antibody (STJ26615) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using RPH3AL antibody (STJ26615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPH3AL antibody (161-315) (STJ26615)

SKU:
STJ26615

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPH3AL (161-315) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPH3AL
Gene ID: 9501
Uniprot ID: RPH3L_HUMAN
Immunogen Region: 161-315
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 161-315 of human RPH3AL (NP_008918.1).
Immunogen Sequence: KYILPLKTPGRADDPHFRPL PTEPAEREPRSSETSRIYTW ARGRVVSSDSDSDSDLSSSS LEDRLPSTGVRDRKGDKPWK ESGGSVEAPRMGFTHPPGHL SGCQSSLASGETGTGSADPP GGPRPGLTRRAPVKDTPGRA PAADAAPAGPSSCLG
Tissue Specificity Moderate to high levels of expression in thyroid, ovary, stomach, heart, pancreas, skeletal muscle, kidney and liver. Also detected in epithelial cells.
Function Rab GTPase effector involved in the late steps of regulated exocytosis, both in endocrine and exocrine cells. Acts as a potential RAB3B effector protein in epithelial cells.
Protein Name Rab Effector Noc2
No C2 Domains Protein
Rabphilin-3a-Like Protein
Cellular Localisation Cytoplasm
Cytoplasmic Vesicle
Secretory Vesicle Membrane
Recruited To The Vesicle Membrane In A Gtp- And Rab3b-Dependent Manner In Epithelial Cells
Alternative Antibody Names Anti-Rab Effector Noc2 antibody
Anti-No C2 Domains Protein antibody
Anti-Rabphilin-3a-Like Protein antibody
Anti-RPH3AL antibody
Anti-NOC2 antibody

Information sourced from Uniprot.org

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