• Western blot analysis of various lysates using RNF181 Rabbit polyclonal antibody (STJ116616) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RNF181 antibody (1-153) (STJ116616)

SKU:
STJ116616

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNF181 (1-153) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF181
Gene ID: 51255
Uniprot ID: RN181_HUMAN
Immunogen Region: 1-153
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human RNF181 (NP_057578.1).
Immunogen Sequence: MASYFDEHDCEPSDPEQETR TNMLLELARSLFNRMDFEDL GLVVDWDHHLPPPAAKTVVE NLPRTVIRGSQAELKCPVCL LEFEEEETAIEMPCHHLFHS SCILPWLSKTNSCPLCRYEL PTDDDTYEEHRRDKARKQQQ QHRLENLHGAMYT
Tissue Specificity Widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletal muscle. Expressed in platelets (at protein level).
Post Translational Modifications Autoubiquitinated as part of the enzymatic reaction.
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Catalyzes monoubiquitination of 26S proteasome subunit PSMC2/RPT1.
Protein Name E3 Ubiquitin-Protein Ligase Rnf181
Ring Finger Protein 181
Database Links Reactome: R-HSA-8866654
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf181 antibody
Anti-Ring Finger Protein 181 antibody
Anti-RNF181 antibody
Anti-HSPC238 antibody

Information sourced from Uniprot.org

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