| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF144A |
| Gene ID: |
9781 |
| Uniprot ID: |
R144A_HUMAN |
| Immunogen Region: |
52-179 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 52-179 of human RNF144A (NP_055561.2). |
| Immunogen Sequence: |
ELLIKEGLETAISCPDAACP KQGHLQENEIECMVAAEIMQ RYKKLQFEREVLFDPCRTWC PASTCQAVCQLQDVGLQTPQ PVQCKACRMEFCSTCKASWH PGQGCPETMPITFLPGETSA AFKMEEDD |
| Post Translational Modifications | Auto-ubiquitinated. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC during DNA damage. Positively regulates DNA virus or exogenous cytosolic DNA-triggered innate immune response by mediating STING1 ubiquitination and increasing its 'Lys-6'-linked ubiquitination and translocation from the endoplasmic reticulum to the Golgi leading to downstream signaling pathways. Plays a positive role in EGF-dependent cell proliferation by prolonging EGF/EGFR signaling during EGF stimulation through EGFR ubiquitination. Increases ERK activity independently of EGFR signaling by promoting polyubiquitination and subsequent degradation of VRK3 in the cytosol. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf144aRing Finger Protein 144aUbcm4-Interacting Protein 4Ubiquitin-Conjugating Enzyme 7-Interacting Protein 4 |
| Database Links | Reactome: R-HSA-8866654 |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinCytoplasmic Vesicle MembraneEndosome MembraneEndoplasmic Reticulum Membrane |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf144a antibodyAnti-Ring Finger Protein 144a antibodyAnti-Ubcm4-Interacting Protein 4 antibodyAnti-Ubiquitin-Conjugating Enzyme 7-Interacting Protein 4 antibodyAnti-RNF144A antibodyAnti-KIAA0161 antibodyAnti-RNF144 antibodyAnti-UBCE7IP4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
