• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g RING1A antibody (STJ11103112). Western blot was performed from the immunoprecipitate using RING1A antibody (STJ11103112) at a dilution of 1:1000.
  • Immunohistochemistry analysis of RING1A in paraffin-embedded human thyroid cancer using RING1A Rabbit monoclonal antibody (STJ11103112) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RING1A Rabbit monoclonal antibody (STJ11103112) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RING1A antibody (100-200) [S2MR] (STJ11103112)

SKU:
STJ11103112

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RING1A (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RING1
Gene ID: 6015
Uniprot ID: RING1_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RING1A A (Q06587).
Immunogen Sequence: DPNFDALISKIYPSREEYEA HQDRVLIRLSRLHNQQALSS SIEEGLRMQAMHRAQRVRRP IPGSDQTTTMSGGEGEPGEG EGDGEDVSSDSAPDSAPGPA P
Function Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
Protein Name E3 Ubiquitin-Protein Ligase Ring1
Polycomb Complex Protein Ring1
Ring Finger Protein 1
Ring-Type E3 Ubiquitin Transferase Ring1
Really Interesting New Gene 1 Protein
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-3108214
Reactome: R-HSA-3899300
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4655427
Reactome: R-HSA-8939243
Reactome: R-HSA-8943724
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Ring1 antibody
Anti-Polycomb Complex Protein Ring1 antibody
Anti-Ring Finger Protein 1 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Ring1 antibody
Anti-Really Interesting New Gene 1 Protein antibody
Anti-RING1 antibody
Anti-RING1A antibody
Anti-RNF1 antibody

Information sourced from Uniprot.org

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