• STJ72805 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72805 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72805 (1µg/ml) staining of HeLa and Mouse D3 (ES) lysates (35µg protein in RIPA buffer). Data obtained from Hisao Masai, Tokyo Metropolitan Institure of Medical Science, Japan. Primary incubation was 1 hour. Detected by chemiluminescence. This data is from a previous batch, not on sale.

Anti-RIF1 antibody (C-Term) (STJ72805)

SKU:
STJ72805-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF
Reactivity: Human/Mouse/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-RIF1 (C-Term) is suitable for use in ELISA and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Expression of the protein seen in the nucleus of NIH3T3 and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: RIF1
Gene ID: 55183
Uniprot ID: RIF1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_060621.3; NP_001171134.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_060621.3; NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.
Immunogen Sequence: RNLQSRWRSPSHEN
Function Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs. In response to DNA damage, interacts with ATM-phosphorylated TP53BP1. Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs. Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs. In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase. Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Promotes NHEJ of dysfunctional telomeres.
Protein Name Telomere-Associated Protein Rif1
Rap1-Interacting Factor 1 Homolog
Database Links Reactome: R-HSA-5693571
Cellular Localisation Nucleus
Chromosome
Telomere
Cytoplasm
Cytoskeleton
Spindle
Following Interaction With Tp53bp1
Recruited To Sites Of Dna Damage
Such As Dsbs
Exhibits Atm- And Tp53bp1-Dependent Localization To Uncapped Or Aberrant Telomeres And To Dna Double Strand Breaks (Dsbs)
Does Not Associate With Normal Telomere Structures
Localizes To Microtubules Of The Midzone Of The Mitotic Spindle During Anaphase
And To Condensed Chromosomes In Telophase
Alternative Antibody Names Anti-Telomere-Associated Protein Rif1 antibody
Anti-Rap1-Interacting Factor 1 Homolog antibody
Anti-RIF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance