• Western blot analysis of lysates from HT-29 cells, using RhoH Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from RAW264.7cells using RhoH antibody.
  • Immunofluorescence analysis of A549 cells, using RhoH Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-RHOH antibody (141-190 aa) (STJ95448)

SKU:
STJ95448

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Rho-related GTP-binding protein RhoH (141-190 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHOH
Gene ID: 399
Uniprot ID: RHOH_HUMAN
Immunogen Region: 141-190 aa
Specificity: Rho H Polyclonal Antibody detects endogenous levels of Rho H protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human RhoH at the amino acid range 141-190
Post Translational Modifications Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development.
Function Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells. Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils.
Protein Name Rho-Related Gtp-Binding Protein Rhoh
Gtp-Binding Protein Ttf
Translocation Three Four Protein
Database Links Reactome: R-HSA-9013407
Cellular Localisation Cytoplasm
Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Colocalizes Together With Zap70 In The Immunological Synapse
Alternative Antibody Names Anti-Rho-Related Gtp-Binding Protein Rhoh antibody
Anti-Gtp-Binding Protein Ttf antibody
Anti-Translocation Three Four Protein antibody
Anti-RHOH antibody
Anti-ARHH antibody
Anti-TTF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance