• Immunofluorescence analysis of L929 cells using RHOG Rabbit polyclonal antibody (STJ11103021) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RHOG in paraffin-embedded human appendix using RHOG Rabbit polyclonal antibody (STJ11103021) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RHOG in paraffin-embedded human colon carcinoma using RHOG Rabbit polyclonal antibody (STJ11103021) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RHOG in paraffin-embedded human liver cancer using RHOG Rabbit polyclonal antibody (STJ11103021) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RHOG Rabbit polyclonal antibody (STJ11103021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RHOG antibody (50-150) (STJ11103021)

SKU:
STJ11103021

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RHOG (50-150) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHOG
Gene ID: 391
Uniprot ID: RHOG_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human RHOG (NP_001656.2).
Immunogen Sequence: TVNLNLWDTAGQEEYDRLRT LSYPQTNVFVICFSIASPPS YENVRHKWHPEVCHHCPDVP ILLVGTKKDLRAQPDTLRRL KEQGQAPITPQQGQALAKQI H
Post Translational Modifications (Microbial infection) Glucosylated at Thr-35 by C.difficile toxin TcdB. (Microbial infection) Glycosylated (O-GlcNAcylated) at Thr-35 by C.novyi toxin TcdA. O-GlcNAcylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption.
Function Plays a role in immunological synaptic F-actin density and architecture organization. Regulates actin reorganization in lymphocytes, possibly through the modulation of Rac1 activity. Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. Binds phospholipids in an activation-dependent manner.thereby acting as an anchor for other proteins to the plasma membrane (PM). Plays a role in exocytosis of cytotoxic granules (CG) by lymphocytes/Component of the exocytosis machinery in natural killer (NK) and CD8+ T cells. Promotes the docking of cytotoxic granules (CG) to the plasma membrane through the interaction with UNC13D. Involved in the cytotoxic activity of lymphocytes/primary CD8+ T cells. (Microbial infection) In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.
Protein Name Rho-Related Gtp-Binding Protein Rhog
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-5625970
Reactome: R-HSA-6798695
Reactome: R-HSA-6811558
Reactome: R-HSA-9013408
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Alternative Antibody Names Anti-Rho-Related Gtp-Binding Protein Rhog antibody
Anti-RHOG antibody
Anti-ARHG antibody

Information sourced from Uniprot.org

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