• Western blot analysis of extracts of various cell lines, using RHBDF1 antibody (STJ119793) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of L929 cells using RHBDF1 antibody (STJ119793) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using RHBDF1 rabbit polyclonal antibody (STJ119793) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RHBDF1 antibody (200-400) (STJ119793)

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STJ119793

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RHBDF1 (200-400) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHBDF1
Gene ID: 64285
Uniprot ID: RHDF1_HUMAN
Immunogen Region: 200-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human RHBDF1 (NP_071895.3).
Immunogen Sequence: HRLPRRRKRESVAKMSFRAA AALMKGRSVRDGTFRRAQRR SFTPASFLEEDTTDFPDELD TSFFAREGILHEELSTYPDE VFESPSEAALKDWEKAPEQA DLTGGALDRSELERSHLMLP LERGWRKQKEGAAAPQPKVR LRQEVVSTAGPRRGQRIAVP VRKLFAREKRPYGLGMVGRL TNRTYRKRIDSFVKRQIEDM D
Tissue Specificity Highly expressed in cerebellum, cerebrum, heart, skeletal muscle, placenta, pancreatic islet and testis. Detected at lower levels in colon, kidney, small intestine and lung.
Post Translational Modifications N-glycosylated.
Function Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.
Protein Name Inactive Rhomboid Protein 1
Irhom1
Epidermal Growth Factor Receptor-Related Protein
Rhomboid 5 Homolog 1
Rhomboid Family Member 1
P100hrho
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Golgi Apparatus Membrane
Predominantly Localized In The Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Inactive Rhomboid Protein 1 antibody
Anti-Irhom1 antibody
Anti-Epidermal Growth Factor Receptor-Related Protein antibody
Anti-Rhomboid 5 Homolog 1 antibody
Anti-Rhomboid Family Member 1 antibody
Anti-P100hrho antibody
Anti-RHBDF1 antibody
Anti-C16orf8 antibody
Anti-DIST1 antibody
Anti-IRHOM1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance