| Host: | Goat |
| Applications: | WB/IF/IHC-P/IHC-F/IEM |
| Reactivity: | RFP/DsRed/mCherry/mOrange/mPlum/mRFP/mStrawberry/tdTomato |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal anti-Red Fluorescent Protein for use in WB, IF, IHC-P, IHC-F and IEM in RFP, DsRed, mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 3 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC-F 1:50-1:500IHC-P 1:50-1:500IF 1:50-1:500ELISA NDTan Z Kong M Wen S et al. J Bone Miner Res 2020 Jul. PMID: 32662900Endo T Kadoya K Suzuki Y et al. Front Cell Neurosci 2019 Jun. PMID: 31316351Jian |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Specificity: | In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein). |
| Immunogen: | Purified recombinant RFP peptide produced in E. coli. |
| Immunogen Sequence: | MVSKGEEDNMAIIKEFMRFK VSKGEEDNMAIIKEFMRFKV SKGEEDNMAIIKEFMRFKVH KGEEDNMAIIKEFMRFKVHM GEEDNMAIIKEFMRFKVHME EEDNMAIIKEFMRFKVHMEG EDNMAIIKEFMRFKVHMEGS DNMAIIKEFMRFKVHMEGSV NMAIIKEFMRFKVHMEGSVN MAIIKEFMRFKVHMEGSVNG AIIKEFMRFKVHMEGSVNGH IIKEFMRFKVHMEGSVNGH |
| Background | Goat polyclonal antibody to RFP (Red fluorescent protein). RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. |
Information sourced from Uniprot.org
