| Host: |
Goat |
| Applications: |
WB/IHC-F/IHC-P/IF/ELISA |
| Reactivity: |
tdTomato/mCherry/RFP |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-Red Fluorescent Protein is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:500-1:2000IHC-F 1:50-1:500IHC-P 1:50-1:500IF 1:50-1:500ELISA NDLorincz R Alvarez AB Walkey CJ et al. Biomed Pharmacother 2022 Dec PMID: 36587560Hirschberg S Dvorzhak A Rasooli-Nejad S et al. bioRxiv 2020Pan H F |
| Storage Instruction: |
For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Specificity: |
In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent p |
| Immunogen: |
Purified recombinant peptide produced in E. coli. |
| Immunogen Sequence: |
MEQKLISEEDLGTISTMVSK GEEDNMAIIKEFMRFKVHME GSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWD ILSPQFMYGSKAYVKHPADI PDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFI YKVKLRGTNFPSDGPVMQKK TMGWEASSERMYPEDGALKG EIKQRLKLKDGGHYDAEVKT TYKAKKPVQLPGAYNVNIKL DITSHNEDYTIVEQYERAE |
| Background | Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. Its brightness and emission wavelength makes it ideal for live animal research. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
