| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RFK |
| Gene ID: | 55312 |
| Uniprot ID: | RIFK_HUMAN |
| Immunogen Region: | 1-155 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-155 of human RFK (NP_060809.3). |
| Immunogen Sequence: | MRHLPYFCRGQVVRGFGRGS KQLGIPTANFPEQVVDNLPA DISTGIYYGWASVGSGDVHK MVVSIGWNPYYKNTKKSMET HIMHTFKEDFYGEILNVAIV GYLRPEKNFDSLESLISAIQ GDIEEAKKRLELPEHLKIKE DNFFQVSKSKIMNGH |
| Tissue Specificity | Detected in brain, placenta and urinary bladder. |
| Function | Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. |
| Protein Name | Riboflavin Kinase Atp -Riboflavin 5'-Phosphotransferase Flavokinase |
| Database Links | Reactome: R-HSA-196843 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Riboflavin Kinase antibody Anti-Atp -Riboflavin 5'-Phosphotransferase antibody Anti-Flavokinase antibody Anti-RFK antibody |
Information sourced from Uniprot.org