Anti-Recombinant-ALKBH2 antibody [RM2S19] (STJA0026577)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Dna Oxidative Demethylase Alkbh2 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2S19 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | WB: 1:500-1:1000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | ALKBH2 |
| Gene ID | 121642 |
| Uniprot ID | ALKB2_HUMAN |
Additional Info
| Function | Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA. Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis. Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine. Acts as a gatekeeper of genomic integrity under alkylation stress. Efficiently repairs alkylated lesions in ribosomal DNA (rDNA). These lesions can cause ss- and dsDNA strand breaks that severely impair rDNA transcription. In a response mechanism to DNA damage, associates with PCNA at replication forks to repair alkylated adducts prior to replication. |
| Protein Name | Dna Oxidative Demethylase Alkbh2Alkylated Dna Repair Protein Alkb Homolog 2Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 2Oxy Dc1 |
| Database Links | Reactome: R-HSA-112122 |
| Cellular Localisation | NucleusNucleolusNucleoplasmRelocates To The Replication Foci During S-Phase |
| Alternative Antibody Names | Anti-Dna Oxidative Demethylase Alkbh2 antibodyAnti-Alkylated Dna Repair Protein Alkb Homolog 2 antibodyAnti-Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 2 antibodyAnti-Oxy Dc1 antibodyAnti-ALKBH2 antibodyAnti-ABH2 antibody |
Information sourced from Uniprot.org