• Western blot analysis of extracts of various cell lines, using NeuN rabbit monoclonal antibody antibody (STJ11101623) at1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using NeuN rabbit monoclonal antibody (STJ11101623) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human brain using NeuN rabbit monoclonal antibody (STJ11101623) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NeuN in paraffin-embedded mouse brain tissue using NeuN rabbit monoclonal antibody (STJ11101623) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of NeuN in paraffin-embedded rat brain tissue using NeuN rabbit monoclonal antibody (STJ11101623) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of mouse brain, using NeuN rabbit monoclonal antibody (STJ11101623) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal imaging of paraffin-embedded mouse brain using NeuN rabbit monoclonal antibody (STJ11101623, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.
  • Confocal imaging of paraffin-embedded rat brain using NeuN rabbit monoclonal antibody (STJ11101623, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

Anti-RBFOX3 antibody (1-100) [S3MR] (STJ11101623)

SKU:
STJ11101623

£146.50 - £639.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-NeuN (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBFOX3
Gene ID: 146713
Uniprot ID: RFOX3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NeuN (A6NFN3).
Immunogen Sequence: MAQPYPPAQYPPPPQNGIPA EYAPPPPHPTQDYSGQTPVP TEHGMTLYTPAQTHPEQPGS EASTQPIAGTQTVPQTDEAA QTDSQPLHPSDPTEKQQPKR
Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
Protein Name Rna Binding Protein Fox-1 Homolog 3
Fox-1 Homolog C
Neuronal Nuclei Antigen
Neun Antigen
Database Links Reactome: R-HSA-9768919
Cellular Localisation Nucleus
Cytoplasm
Largely Restricted To Neuronal Nuclei
However
Significant Cytoplasmic Localization In Neurons From Brains From Hiv-Infected Individuals With Cognitive Impairment
Alternative Antibody Names Anti-Rna Binding Protein Fox-1 Homolog 3 antibody
Anti-Fox-1 Homolog C antibody
Anti-Neuronal Nuclei Antigen antibody
Anti-Neun Antigen antibody
Anti-RBFOX3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance