• Western blot analysis of extracts of various cell lines, using PROX1 rabbit monoclonal antibody (STJ11102413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
  • Immunohistochemistry analysis of PROX1 in paraffin-embedded human placenta using PROX1 Rabbit monoclonal antibody (STJ11102413) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human placenta using PROX1 rabbit monoclonal antibody (STJ11102413) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PROX1 Rabbit monoclonal antibody (STJ11102413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-PROX1 antibody (1-100) [S3MR] (STJ11102413)

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STJ11102413

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PROX1 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PROX1
Gene ID: 5629
Uniprot ID: PROX1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PROX1 (Q92786).
Immunogen Sequence: MPDHDSTALLSRQTKRRRVD IGVKRTVGTASAFFAKARAT FFSAMNPQGSEQDVEYSVVQ HADGEKSNVLRKLLKRANSY EDAMMPFPGATIISQLLKNN
Tissue Specificity Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
Function Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
Protein Name Prospero Homeobox Protein 1
Homeobox Prospero-Like Protein Prox1
Prox-1
Cellular Localisation Nucleus
Rorg Promotes Its Nuclear Localization
Alternative Antibody Names Anti-Prospero Homeobox Protein 1 antibody
Anti-Homeobox Prospero-Like Protein Prox1 antibody
Anti-Prox-1 antibody
Anti-PROX1 antibody

Information sourced from Uniprot.org

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