Anti-RAPGEF1 antibody (844-1095) (STJ118491)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RAPGEF1 (844-1095) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAPGEF1 |
| Gene ID | 2889 |
| Uniprot ID | RPGF1_HUMAN |
| Immunogen Region | 844-1095 |
| Immunogen Sequence | ARGVAARPGTLHDFHSHEIA EQLTLLDAELFYKIEIPEVL LWAKEQNEEKSPNLTQFTEH FNNMSYWVRSIIMLQEKAQD RERLLLKFIKIMKHLRKLNN FNSYLAILSALDSAPIRRLE WQKQTSEGLAEYCTLIDSSS SFRAYRAALSEVEPPCIPYL GLILQDLTFVHLGNPDYIDG KVNFSKRWQQFNILDSMRCF QQAHYDMRRNDDIINFFNDF SDHLAEEALWELSLKIKPR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 844-1095 of human RAPGEF1 (NP_941372.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver. |
| Post Translational Modifications | Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor. |
| Function | Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth. |
| Protein Name | Rap Guanine Nucleotide Exchange Factor 1Crk Sh3-Binding GnrpGuanine Nucleotide-Releasing Factor 2Protein C3g |
| Database Links | Reactome: R-HSA-170968Reactome: R-HSA-186763Reactome: R-HSA-8875555Reactome: R-HSA-9013423Reactome: R-HSA-9027284Reactome: R-HSA-912631 |
| Cellular Localisation | Early Endosome |
| Alternative Antibody Names | Anti-Rap Guanine Nucleotide Exchange Factor 1 antibodyAnti-Crk Sh3-Binding Gnrp antibodyAnti-Guanine Nucleotide-Releasing Factor 2 antibodyAnti-Protein C3g antibodyAnti-RAPGEF1 antibodyAnti-GRF2 antibody |
Information sourced from Uniprot.org