| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAPGEF1 |
| Gene ID: | 2889 |
| Uniprot ID: | RPGF1_HUMAN |
| Immunogen Region: | 844-1095 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 844-1095 of human RAPGEF1 (NP_941372.1). |
| Immunogen Sequence: | ARGVAARPGTLHDFHSHEIA EQLTLLDAELFYKIEIPEVL LWAKEQNEEKSPNLTQFTEH FNNMSYWVRSIIMLQEKAQD RERLLLKFIKIMKHLRKLNN FNSYLAILSALDSAPIRRLE WQKQTSEGLAEYCTLIDSSS SFRAYRAALSEVEPPCIPYL GLILQDLTFVHLGNPDYIDG KVNFSKRWQQFNILDSMRCF QQAHYDMRRNDDIINFFNDF SDHLAEEALWELSLKIKPR |
| Tissue Specificity | Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver. |
| Post Translational Modifications | Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor. |
| Function | Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth. |
| Protein Name | Rap Guanine Nucleotide Exchange Factor 1 Crk Sh3-Binding Gnrp Guanine Nucleotide-Releasing Factor 2 Protein C3g |
| Database Links | Reactome: R-HSA-170968 Reactome: R-HSA-186763 Reactome: R-HSA-8875555 Reactome: R-HSA-9013423 Reactome: R-HSA-9027284 Reactome: R-HSA-912631 |
| Cellular Localisation | Early Endosome |
| Alternative Antibody Names | Anti-Rap Guanine Nucleotide Exchange Factor 1 antibody Anti-Crk Sh3-Binding Gnrp antibody Anti-Guanine Nucleotide-Releasing Factor 2 antibody Anti-Protein C3g antibody Anti-RAPGEF1 antibody Anti-GRF2 antibody |
Information sourced from Uniprot.org
