• Immunofluorescence analysis of U-2 OS cells using RAPGEF1 Rabbit polyclonal antibody (STJ118491) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using RAPGEF1 Rabbit polyclonal antibody (STJ118491) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse liver, using RAPGEF1 Rabbit polyclonal antibody (STJ118491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RAPGEF1 antibody (844-1095) (STJ118491)

SKU:
STJ118491

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAPGEF1
Gene ID: 2889
Uniprot ID: RPGF1_HUMAN
Immunogen Region: 844-1095
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 844-1095 of human RAPGEF1 (NP_941372.1).
Immunogen Sequence: ARGVAARPGTLHDFHSHEIA EQLTLLDAELFYKIEIPEVL LWAKEQNEEKSPNLTQFTEH FNNMSYWVRSIIMLQEKAQD RERLLLKFIKIMKHLRKLNN FNSYLAILSALDSAPIRRLE WQKQTSEGLAEYCTLIDSSS SFRAYRAALSEVEPPCIPYL GLILQDLTFVHLGNPDYIDG KVNFSKRWQQFNILDSMRCF QQAHYDMRRNDDIINFFNDF SDHLAEEALWELSLKIKPR
Tissue Specificity Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
Post Translational Modifications Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor.
Function Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.
Protein Name Rap Guanine Nucleotide Exchange Factor 1
Crk Sh3-Binding Gnrp
Guanine Nucleotide-Releasing Factor 2
Protein C3g
Database Links Reactome: R-HSA-170968
Reactome: R-HSA-186763
Reactome: R-HSA-8875555
Reactome: R-HSA-9013423
Reactome: R-HSA-9027284
Reactome: R-HSA-912631
Cellular Localisation Early Endosome
Alternative Antibody Names Anti-Rap Guanine Nucleotide Exchange Factor 1 antibody
Anti-Crk Sh3-Binding Gnrp antibody
Anti-Guanine Nucleotide-Releasing Factor 2 antibody
Anti-Protein C3g antibody
Anti-RAPGEF1 antibody
Anti-GRF2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance