Anti-RAPGEF1 antibody (470-519 aa) (STJ91934)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RAPGEF1 at the amino acid range 470-519
General Information
| Short Description | Rabbit polyclonal anti-Rap guanine nucleotide exchange factor 1 (470-519 aa) for use in IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAPGEF1 |
| Gene ID | 2889 |
| Uniprot ID | RPGF1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RAPGEF1 at the amino acid range 470-519 |
| Immunogen Region | 470-519 aa |
| Specificity | C3G Polyclonal Antibody detects endogenous levels of C3G protein. |
Additional Info
| Post Translational Modifications | Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor. |
| Function | Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth. |
| Protein Name | Rap Guanine Nucleotide Exchange Factor 1Crk Sh3-Binding GnrpGuanine Nucleotide-Releasing Factor 2Protein C3g |
| Database Links | Reactome: R-HSA-170968Reactome: R-HSA-186763Reactome: R-HSA-8875555Reactome: R-HSA-9013423Reactome: R-HSA-9027284Reactome: R-HSA-912631 |
| Cellular Localisation | Early Endosome |
| Alternative Antibody Names | Anti-Rap Guanine Nucleotide Exchange Factor 1 antibodyAnti-Crk Sh3-Binding Gnrp antibodyAnti-Guanine Nucleotide-Releasing Factor 2 antibodyAnti-Protein C3g antibodyAnti-RAPGEF1 antibodyAnti-GRF2 antibody |
Information sourced from Uniprot.org