• Immunofluorescence analysis of HeLa cells using Rap1B Rabbit polyclonal antibody (STJ114791) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Rap1B Rabbit polyclonal antibody (STJ114791) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-RAP1B antibody (1-184) (STJ114791)

SKU:
STJ114791

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAP1B (1-184) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAP1B
Gene ID: 5908
Uniprot ID: RAP1B_HUMAN
Immunogen Region: 1-184
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human Rap1B (NP_056461.1).
Immunogen Sequence: MREYKLVVLGSGGVGKSALT VQFVQGIFVEKYDPTIEDSY RKQVEVDAQQCMLEILDTAG TEQFTAMRDLYMKNGQGFAL VYSITAQSTFNDLQDLREQI LRVKDTDDVPMILVGNKCDL EDERVVGKEQGQNLARQWNN CAFLESSAKSKINVNEIFYD LVRQINRKTPVPGKARKKSS CQLL
Function GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function.
Protein Name Ras-Related Protein Rap-1b
Gtp-Binding Protein Smg P21b
Database Links Reactome: R-HSA-354192
Reactome: R-HSA-354194
Reactome: R-HSA-372708
Reactome: R-HSA-392517
Reactome: R-HSA-5674135
Reactome: R-HSA-6798695
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-8875555
Reactome: R-HSA-8950505
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Cellular Localisation Cell Membrane
Cytoplasm
Cytosol
Cell Junction
May Shuttle Between Plasma Membrane And Cytosol
Presence Of Krit1 And Cdh5 Is Required For Its Localization To The Cell Junction
Alternative Antibody Names Anti-Ras-Related Protein Rap-1b antibody
Anti-Gtp-Binding Protein Smg P21b antibody
Anti-RAP1B antibody
Anti-OK antibody
Anti-SW-cl.11 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance