| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAP1B |
| Gene ID: | 5908 |
| Uniprot ID: | RAP1B_HUMAN |
| Immunogen Region: | 1-184 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human Rap1B (NP_056461.1). |
| Immunogen Sequence: | MREYKLVVLGSGGVGKSALT VQFVQGIFVEKYDPTIEDSY RKQVEVDAQQCMLEILDTAG TEQFTAMRDLYMKNGQGFAL VYSITAQSTFNDLQDLREQI LRVKDTDDVPMILVGNKCDL EDERVVGKEQGQNLARQWNN CAFLESSAKSKINVNEIFYD LVRQINRKTPVPGKARKKSS CQLL |
| Function | GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function. |
| Protein Name | Ras-Related Protein Rap-1bGtp-Binding Protein Smg P21b |
| Database Links | Reactome: R-HSA-354192Reactome: R-HSA-354194Reactome: R-HSA-372708Reactome: R-HSA-392517Reactome: R-HSA-5674135Reactome: R-HSA-6798695Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-8875555Reactome: R-HSA-8950505Reactome: R-HSA-9649948Reactome: R-HSA-9656223 |
| Cellular Localisation | Cell MembraneCytoplasmCytosolCell JunctionMay Shuttle Between Plasma Membrane And CytosolPresence Of Krit1 And Cdh5 Is Required For Its Localization To The Cell Junction |
| Alternative Antibody Names | Anti-Ras-Related Protein Rap-1b antibodyAnti-Gtp-Binding Protein Smg P21b antibodyAnti-RAP1B antibodyAnti-OK antibodyAnti-SW-cl.11 antibody |
Information sourced from Uniprot.org