| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RALBP1 (396-655) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RALBP1 |
| Gene ID: |
10928 |
| Uniprot ID: |
RBP1_HUMAN |
| Immunogen Region: |
396-655 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 396-655 of human RALBP1 (NP_006779.1). |
| Immunogen Sequence: |
AGIKEEIRRQEFLLNCLHRD LQGGIKDLSKEERLWEVQRI LTALKRKLREAKRQECETKI AQEIASLSKEDVSKEEMNEN EEVINILLAQENEILTEQEE LLAMEQFLRRQIASEKEEIE RLRAEIAEIQSRQQHGRSET EEYSSESESESEDEEELQII LEDLQRQNEELEIKNNHLNQ AIHEEREAIIELRVQLRLLQ MQRAKAEQQAQEDEEPEWRG GAVQPPRDGVLEPKAAKEQ |
| Tissue Specificity | Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes. |
| Post Translational Modifications | Tyrosine-phosphorylated upon stimulation of cells with EGF. May undergo proteolytic cleavage to give peptides which reassemble to form a transporter complex. |
| Function | Multifunctional protein that functions as a downstream effector of RALA and RALB. As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity. As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis. During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle. During mitosis, also controls mitochondrial fission as an effector of RALA. Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L. Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin (DOX) contributing to cell multidrug resistance. |
| Protein Name | Rala-Binding Protein 1Ralbp176 Kda Ral-Interacting ProteinDinitrophenyl S-Glutathione AtpaseDnp-Sg AtpaseRal-Interacting Protein 1 |
| Database Links | Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmCytosolCytoskeletonSpindle PoleNucleusMitochondrionCytosolic Protein That Transiently Associates With The Mitotic Spindle Poles In Early ProphaseAnd Dissociates From Them After Completion Of MitosisTargeted To The Plasma Membrane Through Its Interaction With RalbDirected By Fgf SignalingDocking On The Membrane Is Required To Transduce The Ral SignalRecruited By Rala To The Mitochondrion During Mitosis Where It Regulates Mitochondrial FissionNuclear Localization Is Cell Cycle Dependent While Membrane Localization Is Seen In Adherent CellsThe Region Involved In Membrane Association Could Form Transmembrane Domains And Expose A Part Of The Protein Extracellularly (Probable) |
| Alternative Antibody Names | Anti-Rala-Binding Protein 1 antibodyAnti-Ralbp1 antibodyAnti-76 Kda Ral-Interacting Protein antibodyAnti-Dinitrophenyl S-Glutathione Atpase antibodyAnti-Dnp-Sg Atpase antibodyAnti-Ral-Interacting Protein 1 antibodyAnti-RALBP1 antibodyAnti-RLIP antibodyAnti-RLIP1 antibodyAnti-RLIP76 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
