| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-RAC2 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 22kDa band observed in lysates of cell line Molt4 (calculated MW of 21.4kDa according to NP_002863.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
RAC2 |
| Gene ID: |
5880 |
| Uniprot ID: |
RAC2_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_002863.1 |
| Immunogen Sequence: |
PQPTRQQKRACSLL |
| Post Translational Modifications | (Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly. |
| Function | Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. |
| Protein Name | Ras-Related C3 Botulinum Toxin Substrate 2GxSmall G ProteinP21-Rac2 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-1222556Reactome: R-HSA-1257604Reactome: R-HSA-2219530Reactome: R-HSA-4086400Reactome: R-HSA-5668599Reactome: R-HSA-6811558Reactome: R-HSA-9013404 |
| Cellular Localisation | CytoplasmMembrane-Associated When Activated |
| Alternative Antibody Names | Anti-Ras-Related C3 Botulinum Toxin Substrate 2 antibodyAnti-Gx antibodyAnti-Small G Protein antibodyAnti-P21-Rac2 antibodyAnti-RAC2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
