• Anti-Rab5b antibody at 1:500 dilution 293 cells transfected with GFP-Rab5 lysates at 50 µg per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Anti-Rab5b antibody at 1:500 dilution lysates at 50 µg per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunohistochemistry of mouse stomach using anti-Rab5b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5b antibody at 1:500:DAB detection.
  • Immunohistochemistry of mouse retina using anti-Rab5b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5b antibody at 1:500:DAB detection.
  • Immunohistochemistry of mouse pancreas using anti-Rab5b antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5b antibody at 1:250:DAB detection.
  • Immunofluorescence – anti-Rab5b antibody using B6-RPE07 cells at 1:100 dilution

Anti-RAB5B antibody (115aa C-Term) (STJ140061)

SKU:
STJ140061

£100.50 - £393.50
Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-RAB5B, member RAS oncogene family (115aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 4 mg/ml
Dilution Range: WB 1:250-1:1000
IF 1:50-1:250
IHC-F 1:100-1:500
IHC-P 1:100-1:500
Cardoso MHS PhD Thesis NOVA University of Lisbon Portugal 2018
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Gene Symbol: RAB5B
Gene ID: 5869
Uniprot ID: RAB5B_HUMAN
Immunogen Region: 115aa C-Term
Accession Number: ENSG00000111540
Specificity: Detects Rab5b protein by Western blot in cell lysates of transfected cells with GFP-Rab5b. This Ab is specific for Rab5b; it does not recognize Rab5a and Rab5c.
Immunogen: Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5b produced in E. coli.
Post Translational Modifications Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2. (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.
Function Protein transport. Probably involved in vesicular traffic.
Protein Name Ras-Related Protein Rab-5b
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8854214
Reactome: R-HSA-8856828
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Early Endosome Membrane
Melanosome
Enriched In Stage I Melanosomes
Alternative Antibody Names Anti-Ras-Related Protein Rab-5b antibody
Anti-RAB5B antibody

Information sourced from Uniprot.org

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