| Host: | Goat |
| Applications: | WB/IHC-F/IHC-P |
| Reactivity: | Human/Rat/Mouse/Canine/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal anti-RAB3, member RAS oncogene family (181aa C-Term) for use in WB, IHC-F and IHC-P in Human, Rat, Mouse, Canine and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 2 mg/mL |
| Dilution Range: | WB 1:1000-1:2000IHC-F 1:250-1:1000IHC-P 1:250-1:1000Cabaço LC Bento-Lopes L Neto MV et al. JID Innov 2022 Jun PMID: 36090299 |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: | RAB3A |
| Gene ID: | 5864 |
| Uniprot ID: | RAB3A_HUMAN |
| Immunogen Region: | 181aa C-Term |
| Accession Number: | ENSG00000105649 |
| Specificity: | Detects endogenous levels of Rab3a by Western blot. It does not cross-react with Rab3b, Rab3c and Rab3c. |
| Immunogen: | Recombinant peptide derived from within residues 181 aa to the C-terminus of Rab3a produced in E. coli. |
| Immunogen Sequence: | EFMSTQIKTYSWDNAQVLLV FMSTQIKTYSWDNAQVLLVG MSTQIKTYSWDNAQVLLVGN STQIKTYSWDNAQVLLVGNK TQIKTYSWDNAQVLLVGNKC QIKTYSWDNAQVLLVGNKCD |
| Tissue Specificity | Specifically expressed in brain. |
| Post Translational Modifications | Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB3A plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT. |
| Protein Name | Ras-Related Protein Rab-3a |
| Database Links | Reactome: R-HSA-181429Reactome: R-HSA-181430Reactome: R-HSA-210500Reactome: R-HSA-212676Reactome: R-HSA-264642Reactome: R-HSA-6798695Reactome: R-HSA-8873719Reactome: R-HSA-8876198Reactome: R-HSA-888590Reactome: R-HSA-9662360 |
| Cellular Localisation | CytoplasmCytosolLysosomeCytoplasmic VesicleSecretory VesicleCell ProjectionAxonCell MembraneLipid-AnchorCytoplasmic SidePresynapsePostsynapseCycles Between A Vesicle-Associated Gtp-Bound Form And A Cytosolic Gdp-Bound Form |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-3a antibodyAnti-RAB3A antibody |
Information sourced from Uniprot.org
