Anti-RAB39A antibody (50-130 aa) (STJ191283)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 50-130
General Information
| Short Description | Rabbit polyclonal anti-Ras-related protein Rab-39A (50-130 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAB39A |
| Gene ID | 54734 |
| Uniprot ID | RB39A_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 50-130 |
| Immunogen Region | 50-130 aa |
| Specificity | RB39A Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB39A regulates autophagosome-lysosome fusion via recruitment of the HOPS endosomal tethering complex onto lysosomes.this process involves lysosomal RAB39A and autophagosomal RAB2A recruitment of HOPS subcomplexes VPS41-VPS16-VPS18-VPS33A and VPS39-VPS11, respectively, which assemble into a functional complex to mediate membrane tethering and SNAREs-driven membrane fusion. Also negatively regulates lipopolysaccharide (LPS)-induced autophagosome formation in macrophages, possibly by implicating PI3K. Promotes the delivery of MHC-I molecules from the ER to phagosomes and the generation of peptide-loaded MHC-I complexes in phagosomes, thus enhancing antigen cross-presentation by dendritic cells. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. May be involved in multiple neurite formation. |
| Protein Name | Ras-Related Protein Rab-39aRab-39 |
| Database Links | Reactome: R-HSA-6811438Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideCytoplasmic VesiclePhagosome MembraneLysosome MembraneAutolysosome MembraneRecruited To Phagosomes Containing SAureus Or MTuberculosisMajorly Localized On Lysosomes Under Basal ConditionsMajorly Localized On Autophagosomes/Autolysosomes Under Autophagy-Induced ConditionsColocalized With Vps39 And Vps41 On Lysosomes Or AutolysosomesColocalized With Stx17 On Autolysosomes In Autophagy-Induced ConditionsLocalized (Gtp-Bound) To Phagosomes In Dendritic CellsShuttles Vesicles From The Er-Golgi To The Phagosome |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-39a antibodyAnti-Rab-39 antibodyAnti-RAB39A antibodyAnti-RAB39 antibody |
Information sourced from Uniprot.org