| Host: | Goat |
| Applications: | WB/IF |
| Reactivity: | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal anti-RAB28, member RAS oncogene family (120aa C-Term) for use in WB and IF in Human, Mouse, Rat, Bovine, Canine, Chicken, Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep and Simian samples. Datasheet in |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 3 mg/mL |
| Dilution Range: | WB 1:250-1:2000IF 1:25-1:250 |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: | RAB28 |
| Gene ID: | 9364 |
| Uniprot ID: | RAB28_HUMAN |
| Immunogen Region: | 120aa C-Term |
| Accession Number: | ENSG00000157869 |
| Specificity: | Detects Rab28 by Western blot in transfected cells with GFP-Rab28. |
| Immunogen: | Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab28 produced in E. coli. |
| Immunogen Sequence: | MSETQPLVALVGNKIDLEHM SETQPLVALVGNKIDLEHMR ETQPLVALVGNKIDLEHMRT TQPLVALVGNKIDLEHMRTV QPLVALVGNKIDLEHMRTVK PLVALVGNKIDLEHMRTVKA |
| Tissue Specificity | Isoform S: Isoform S is detected in most tissues investigated: cortex, liver, kidney, skeletal muscle, adipose tissue, testis, urothelium, lung, bone marrow and retinal pigment epithelium (RPE). Isoform L: Isoform L 2 is widely and abundantly expressed all tissues. Isoform 3: Isoform 3 is highly expressed in heart, lung, bone marrow, retina, brain, and RPE. |
| Post Translational Modifications | Isoprenylated. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB28 is required for shedding and phagocytosis of cone cell outer segments (OS) discs in the retina. Also participates in nuclear factor kappa-B p65/RELA nuclear transport in endothelial cells. |
| Protein Name | Ras-Related Protein Rab-28 |
| Database Links | |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideCytoplasmCytoskeletonCilium Basal BodyNucleusExpressed In The Basal Body And Ciliary Rootlet Of The Photoreceptor CellsLocalized In The Cytoplasm And The Nucleus Of Vascular Endothelial Cells |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-28 antibodyAnti-RAB28 antibody |
Information sourced from Uniprot.org
