• Immunofluorescence analysis of HepG2 cells using [KD Validated] RAB27A Rabbit polyclonal antibody (STJ25258) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and RAB27A knockdown (KD) U-87MG (KD) cells, using [KD Validated] RAB27A Rabbit polyclonal antibody (STJ25258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RAB27A antibody (1-221) (STJ25258)

SKU:
STJ25258

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB27A (1-221) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB27A
Gene ID: 5873
Uniprot ID: RB27A_HUMAN
Immunogen Region: 1-221
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human RAB27A (NP_899057.1).
Immunogen Sequence: MSDGDYDYLIKFLALGDSGV GKTSVLYQYTDGKFNSKFIT TVGIDFREKRVVYRASGPDG ATGRGQRIHLQLWDTAGQER FRSLTTAFFRDAMGFLLLFD LTNEQSFLNVRNWISQLQMH AYCENPDIVLCGNKSDLEDQ RVVKEEEAIALAEKYGIPYF ETSAANGTNISQAIEMLLDL IMKRMERCVDKSWIPEGVVR SNGHASTDQLSEEKEKGACG C
Tissue Specificity Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
Function Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Protein Name Ras-Related Protein Rab-27a
Rab-27
Gtp-Binding Protein Ram
Database Links Reactome: R-HSA-264876
Reactome: R-HSA-6798695
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Membrane
Lipid-Anchor
Melanosome
Late Endosome
Lysosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Localizes To Endosomal Exocytic Vesicles
Alternative Antibody Names Anti-Ras-Related Protein Rab-27a antibody
Anti-Rab-27 antibody
Anti-Gtp-Binding Protein Ram antibody
Anti-RAB27A antibody
Anti-RAB27 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance