Anti-RAB24 antibody (90-170 aa) (STJ95288)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from Rab 24 at the amino acid range 90-170
General Information
| Short Description | Rabbit polyclonal anti-Ras-related protein Rab-24 (90-170 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAB24 |
| Gene ID | 53917 |
| Uniprot ID | RAB24_HUMAN |
| Immunogen | Synthesized peptide derived from Rab 24 at the amino acid range 90-170 |
| Immunogen Region | 90-170 aa |
| Specificity | Rab 24 Polyclonal Antibody detects endogenous levels of Rab 24 protein. |
Additional Info
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB24 is an atypical RAB protein that presents low GTPase activity and thereby exists predominantly in the GTP-bound active state. RAB24 is required for the clearance of late autophagic vacuoles under basal conditions. It is not needed for starvation-induced autophagy. Involved in the modulation of meiotic apparatus assembly and meiotic progression during oocyte maturation, possibly through regulation of kinetochore-microtubule interaction. |
| Protein Name | Ras-Related Protein Rab-24 |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-8873719 |
| Cellular Localisation | CytoplasmCytosolMembraneLipid-AnchorCytoplasmic VesicleAutophagosome MembranePerinuclear RegionCytoskeletonSpindleOnly About 20% Is Recovered In The Particulate FractionRab24 Localizes In Perinuclear Region And In The Limiting Membranes Of Autophagic Compartments Under Basal ConditionsRab24 Is Localized In The Cytoplasm With An Accumulated Distribution In Nuclear Region At Germinal Vesicle (Gv) Stage Of Oocyte Meiotic ProgressionAt Pre-Metaphase I StageLocalized In The Cytoplasm With A Particular Concentration Around ChromosomesAs The Oocytes Enter Metaphase ILocated To The Spindle RegionSimilar Distribution Pattern Is Observed In Mii Oocytes |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-24 antibodyAnti-RAB24 antibody |
Information sourced from Uniprot.org