| Host: |
Goat |
| Applications: |
WB/IHC-F/IHC-P/IF |
| Reactivity: |
Human/Rat/Mouse/Monkey/Canine |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-RAB1, member RAS oncogene family (110aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:250-1:5000IF 1:100-1:500IHC-F 1:250-1:1000IHC-P 1:250-1:1000 |
| Storage Instruction: |
Store at-20 C for long-term storage. Store at 2-8 C for up to one month. |
| Gene Symbol: |
RAB1B |
| Gene ID: |
81876 |
| Uniprot ID: |
RAB1B_HUMAN |
| Immunogen Region: |
110aa C-Term |
| Accession Number: |
ENSG00000174903 |
| Specificity: |
Detects endogenous levels of Rab1b protein by Western blot in whole cell lysates and transfected cells with GFP-Rab1b cds. |
| Immunogen: |
Purified recombinant peptide derived from within residues 110 aa to the C-terminus of Rab1b produced in E. coli. |
| Immunogen Sequence: |
QEIDRYASENVNKLLVGNKS DLTTKKVVDNTTAKEFADSL GVPFLETSAKNATNVEQAFM TMAAEIKKRMGPGAASGGER PNLKIDSTPVKPASGGCC |
| Post Translational Modifications | Prenylated.by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1). (Microbial infection) AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active. It is later de-AMPylated by L.pneumophila SidD, releasing RAB1B from bacterial phagosomes. (Microbial infection) Phosphocholinated at Ser-76 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB. (Microbial infection) Glycosylated by S.typhimurium protein Ssek3: arginine GlcNAcylation prevents GTPase activity, thereby disrupting vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Required to modulate the compacted morphology of the Golgi. Promotes the recruitment of lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment. |
| Protein Name | Ras-Related Protein Rab-1b |
| Database Links | Reactome: R-HSA-162658Reactome: R-HSA-204005Reactome: R-HSA-6807878Reactome: R-HSA-6811434Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | CytoplasmMembraneLipid-AnchorCytoplasmic SidePreautophagosomal Structure MembranePerinuclear RegionTargeted By Rep1 To Membranes Of Specific Subcellular Compartments Including Endoplasmic ReticulumGolgi ApparatusAnd Intermediate Vesicles Between These Two CompartmentsIn The Gdp-FormColocalizes With Gdi In The CytoplasmCo-Localizes With Mtmr6 To The Endoplasmic Reticulum-Golgi Intermediate Compartment And To The Peri-Golgi Region |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-1b antibodyAnti-RAB1B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
