| Function | Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product. Modification of cytoplasmic tRNAs with queuosine controls the elongation speed of cognate codons, thereby ensuring the correct folding of nascent proteins to maintain proteome integrity. |
| Protein Name | Queuine Trna-Ribosyltransferase Catalytic Subunit 1Guanine Insertion EnzymeTrna-Guanine Transglycosylase |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | CytoplasmMitochondrion Outer MembranePeripheral Membrane ProteinCytoplasmic SideWeakly Associates With MitochondriaPossibly Via Qtrt2 |
| Alternative Antibody Names | Anti-Queuine Trna-Ribosyltransferase Catalytic Subunit 1 antibodyAnti-Guanine Insertion Enzyme antibodyAnti-Trna-Guanine Transglycosylase antibodyAnti-QTRT1 antibodyAnti-TGT antibodyAnti-TGUT antibody |
Information sourced from Uniprot.org
