| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Queuine Trna-Ribosyltransferase Catalytic Subunit 1 is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4 |
| Purification: |
Antigen affinity purification |
| Concentration: |
1.08 mg/mL |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Gene Symbol: |
QTRT1 |
| Gene ID: |
81890 |
| Uniprot ID: |
TGT_HUMAN |
| Immunogen: |
Fusion protein of human QTRT1 |
| Function | Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product. |
| Protein Name | Queuine Trna-Ribosyltransferase Catalytic Subunit 1Guanine Insertion EnzymeTrna-Guanine Transglycosylase |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | CytoplasmMitochondrion Outer MembranePeripheral Membrane ProteinCytoplasmic SideWeakly Associates With MitochondriaPossibly Via Qtrt2 |
| Alternative Antibody Names | Anti-Queuine Trna-Ribosyltransferase Catalytic Subunit 1 antibodyAnti-Guanine Insertion Enzyme antibodyAnti-Trna-Guanine Transglycosylase antibodyAnti-QTRT1 antibodyAnti-TGT antibodyAnti-TGUT antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
