Western blot analysis of extracts of U-87MG cells, using PUS1 antibody (STJ116327) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PUS1 antibody (308-427) (STJ116327)

SKU:
STJ116327
£112.50 - £524.50
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PUS1
Gene ID: 80324
Uniprot ID: PUS1_HUMAN
Immunogen Region: 308-427
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 308-427 of human PUS1 (NP_079491.2).
Immunogen Sequence: YAPESVLERSWGTEKVDVPK APGLGLVLERVHFEKYNQRF GNDGLHEPLDWAQEEGKVAA FKEEHIYPTIIGTERDERSM AQWLSTLPIHNFSATALTAG GTGAKVPSPLEGSEGDGDTD
Tissue Specificity Widely expressed. High levels of expression found in brain and skeletal muscle.
Function Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions. Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing. Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA.
Protein Name Pseudouridylate Synthase 1 Homolog
Trna Pseudouridine Synthase 1
Trna Pseudouridine(38-40 Synthase
Trna Pseudouridylate Synthase I
Trna-Uridine Isomerase I
Database Links Reactome: R-HSA-6782315 Q9Y606-2
Reactome: R-HSA-6787450 Q9Y606-1
Cellular Localisation Isoform 1: Mitochondrion
Isoform 2: Nucleus
Cytoplasm
Alternative Antibody Names Anti-Pseudouridylate Synthase 1 Homolog antibody
Anti-Trna Pseudouridine Synthase 1 antibody
Anti-Trna Pseudouridine(38-40 Synthase antibody
Anti-Trna Pseudouridylate Synthase I antibody
Anti-Trna-Uridine Isomerase I antibody
Anti-PUS1 antibody
Anti-PP8985 antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJA0008127 Anti-PUS1 antibody (STJA0008127) Rabbit Polyclonal Human/Mouse/Rat WB
STJ119893 Anti-TSPY3 antibody (1-308) (STJ119893) Rabbit Polyclonal Rat WB/ELISA
STJ28543 Anti-SAP30BP antibody (1-308) (STJ28543) Rabbit Polyclonal Human/Mouse/Rat WB/IHC-P/ELISA
STJ29173 Anti-LDLRAP1 antibody (59-308) (STJ29173) Rabbit Polyclonal Human/Mouse/Rat WB/IF/ICC/ELISA
STJ29696 Anti-IFNGR2 antibody (308-322) (STJ29696) Rabbit Polyclonal Human/Mouse/Rat WB/ELISA
STJ11105532 Anti-GDF15 antibody (197-308) (STJ11105532) Rabbit Polyclonal Human IF/ICC/ELISA
STJ29268 Anti-GTF2H3 antibody (1-308) (STJ29268) Rabbit Polyclonal Human/Mouse WB/IP/ELISA
STJ23764 Anti-GDF15 antibody (49-308) (STJ23764) Rabbit Polyclonal Human/Mouse/Rat WB/IHC-P/IF/ICC/IP/ELISA
!