Western blot analysis of extracts of various cell lines, using PTPN4 antibody (STJ26538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-PTPN4 antibody (1-240) (STJ26538)

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STJ26538
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPN4
Gene ID: 5775
Uniprot ID: PTN4_HUMAN
Immunogen Region: 1-240
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PTPN4 (NP_002821.1).
Immunogen Sequence: MTSRFRLPAGRTYNVRASEL ARDRQHTEVVCNILLLDNTV QAFKVNKHDQGQVLLDVVFK HLDLTEQDYFGLQLADDSTD NPRWLDPNKPIRKQLKRGSP YSLNFRVKFFVSDPNKLQEE YTRYQYFLQIKQDILTGRLP CPSNTAALLASFAVQSELGD YDQSENLSGYLSDYSFIPNQ PQDFEKEIAKLHQQHIGLSP AEAEFNYLNTARTLELYGVE FHYARDQSNNEIMIGVMSG
Post Translational Modifications Highly phosphorylated on serine and threonine residues but not on tyrosines. Cleaved and activated by calpain I/CAPN1.
Function Phosphatase that plays a role in immunity, learning, synaptic plasticity or cell homeostasis. Regulates neuronal cell homeostasis by protecting neurons against apoptosis. Negatively regulates TLR4-induced interferon beta production by dephosphorylating adapter TICAM2 and inhibiting subsequent TRAM-TRIF interaction. Dephosphorylates also the immunoreceptor tyrosine-based activation motifs/ITAMs of the TCR zeta subunit and thereby negatively regulates TCR-mediated signaling pathway. May act at junctions between the membrane and the cytoskeleton.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type 4
Protein-Tyrosine Phosphatase Meg1
Meg
Ptpase-Meg1
Database Links Reactome: R-HSA-166016
Reactome: R-HSA-9008059
Reactome: R-HSA-9022699
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 4 antibody
Anti-Protein-Tyrosine Phosphatase Meg1 antibody
Anti-Meg antibody
Anti-Ptpase-Meg1 antibody
Anti-PTPN4 antibody

Information sourced from Uniprot.org

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