• Immunofluorescence analysis of PC-12 cells using SHP2 Rabbit polyclonal antibody (STJ25224) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using SHP2 antibody (STJ25224) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using SHP2 antibody (STJ25224) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using SHP2 antibody (STJ25224) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SHP2 antibody (STJ25224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PTPN11 antibody (514-593) (STJ25224)

SKU:
STJ25224

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PTPN11 (514-593) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPN11
Gene ID: 5781
Uniprot ID: PTN11_HUMAN
Immunogen Region: 514-593
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 514-593 of human SHP2 (NP_002825.3).
Immunogen Sequence: IYMAVQHYIETLQRRIEEEQ KSKRKGHEYTNIKYSLADQT SGDQSPLPPCTPTPPCAEMR EDSARVYENVGLMQQQKSFR
Tissue Specificity Widely expressed, with highest levels in heart, brain, and skeletal muscle.
Post Translational Modifications Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation.which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA. Phosphorylated by activated PDGFRB.
Function Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. Dephosphorylates CDC73. Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification. Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type 11
Protein-Tyrosine Phosphatase 1d
Ptp-1d
Protein-Tyrosine Phosphatase 2c
Ptp-2c
Sh-Ptp2
Shp-2
Shp2
Sh-Ptp3
Database Links Reactome: R-HSA-1059683
Reactome: R-HSA-109704
Reactome: R-HSA-110056
Reactome: R-HSA-112411
Reactome: R-HSA-114604
Reactome: R-HSA-1170546
Reactome: R-HSA-1257604
Reactome: R-HSA-1295596
Reactome: R-HSA-1433557
Reactome: R-HSA-180292
Reactome: R-HSA-186763
Reactome: R-HSA-210990
Reactome: R-HSA-210993
Reactome: R-HSA-2219530
Reactome: R-HSA-2586552
Reactome: R-HSA-388841
Reactome: R-HSA-389513
Reactome: R-HSA-389948
Reactome: R-HSA-391160
Reactome: R-HSA-418886
Reactome: R-HSA-432142
Reactome: R-HSA-512988
Reactome: R-HSA-5654689
Reactome: R-HSA-5654693
Reactome: R-HSA-5654695
Reactome: R-HSA-5654700
Reactome: R-HSA-5654706
Reactome: R-HSA-5654710
Reactome: R-HSA-5654712
Reactome: R-HSA-5654720
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-6811558
Reactome: R-HSA-877312
Reactome: R-HSA-8853659
Reactome: R-HSA-8854691
Reactome: R-HSA-8865999
Reactome: R-HSA-8934593
Reactome: R-HSA-9008059
Reactome: R-HSA-9028731
Reactome: R-HSA-909733
Reactome: R-HSA-912694
Reactome: R-HSA-936964
Reactome: R-HSA-9607240
Reactome: R-HSA-9645135
Reactome: R-HSA-9674555
Reactome: R-HSA-9680350
Reactome: R-HSA-9702518
Reactome: R-HSA-9703648
Reactome: R-HSA-9705671
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 11 antibody
Anti-Protein-Tyrosine Phosphatase 1d antibody
Anti-Ptp-1d antibody
Anti-Protein-Tyrosine Phosphatase 2c antibody
Anti-Ptp-2c antibody
Anti-Sh-Ptp2 antibody
Anti-Shp-2 antibody
Anti-Shp2 antibody
Anti-Sh-Ptp3 antibody
Anti-PTPN11 antibody
Anti-PTP2C antibody
Anti-SHPTP2 antibody

Information sourced from Uniprot.org

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