||WB, IHC, IF
||Human, Mouse, Rat
||Rabbit polyclonal anti-PTPN1 antibody is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
||FOR RESEARCH USE ONLY (RUO).
||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
||Store at-20°C. Avoid freeze/thaw cycles.
||WB: 1:500-1:2000IHC: 1:50-1:200IF: 1:10-1:100
||Recombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTPN1 (NP_002818.1).
| Tissue Specificity || Expressed in keratinocytes (at protein level). |
| Post Translational Modifications || Oxidized on Cys-215.the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50. S-nitrosylation of Cys-215 inactivates the enzyme activity. Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity. |
| Function || Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK.inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. |
| Protein Name || Tyrosine-Protein Phosphatase Non-Receptor Type 1Protein-Tyrosine Phosphatase 1bPtp-1b |
| Database Links || Reactome: R-HSA-354192Reactome: R-HSA-6807004Reactome: R-HSA-877312Reactome: R-HSA-8849472Reactome: R-HSA-9022699Reactome: R-HSA-912694Reactome: R-HSA-982772 |
| Cellular Localisation || Endoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideInteracts With Epha3 At The Cell Membrane |
| Alternative Antibody Names || Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 1 antibodyAnti-Protein-Tyrosine Phosphatase 1b antibodyAnti-Ptp-1b antibodyAnti-PTPN1 antibodyAnti-PTP1B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance