• Immunofluorescence analysis of MCF-7 cells using PTPN1 antibody (STJ28044). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse brain using PTPN1 antibody (STJ28044) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts from normal (control) and PTPN1 knockout (KO) HeLa cells, using PTPN1 antibody (STJ28044) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.
  • Immunohistochemistry of paraffin-embedded rat brain using PTPN1 antibody (STJ28044) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using PTPN1 antibody (STJ28044) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-PTPN1 antibody (STJ28044)

SKU:
STJ28044

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-PTPN1 antibody is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:10-1:100
Uniprot ID: PTN1_HUMAN
Gene ID: 5770
Gene Symbol: PTPN1
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTPN1 (NP_002818.1).
Tissue Specificity Expressed in keratinocytes (at protein level).
Post Translational Modifications Oxidized on Cys-215.the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50. S-nitrosylation of Cys-215 inactivates the enzyme activity. Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
Function Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK.inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type 1
Protein-Tyrosine Phosphatase 1b
Ptp-1b
Database Links Reactome: R-HSA-354192
Reactome: R-HSA-6807004
Reactome: R-HSA-877312
Reactome: R-HSA-8849472
Reactome: R-HSA-9022699
Reactome: R-HSA-912694
Reactome: R-HSA-982772
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Interacts With Epha3 At The Cell Membrane
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 1 antibody
Anti-Protein-Tyrosine Phosphatase 1b antibody
Anti-Ptp-1b antibody
Anti-PTPN1 antibody
Anti-PTP1B antibody

Information sourced from Uniprot.org

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