• Immunohistochemistry of paraffin-embedded human stomach using PTK7 antibody (STJ111881) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse brain using PTK7 antibody (STJ111881) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human prostate using PTK7 antibody (STJ111881) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using PTK7 antibody (STJ111881) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.

Anti-PTK7 antibody (STJ111881)

SKU:
STJ111881

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Host: Rabbit
Applications: WB, IHC
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-PTK7 antibody is suitable for use in Western Blot and Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:100
Uniprot ID: PTK7_HUMAN
Gene ID: 5754
Gene Symbol: PTK7
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 811-1070 of human PTK7 (NP_002812.2).
Tissue Specificity Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
Post Translational Modifications MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway.
Function Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
Protein Name Inactive Tyrosine-Protein Kinase 7
Colon Carcinoma Kinase 4
Cck-4
Protein-Tyrosine Kinase 7
Pseudo Tyrosine Kinase Receptor 7
Tyrosine-Protein Kinase-Like 7
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Colocalizes With Mmp14 At Cell Junctions
Also Localizes At The Leading Edge Of Migrating Cells
Alternative Antibody Names Anti-Inactive Tyrosine-Protein Kinase 7 antibody
Anti-Colon Carcinoma Kinase 4 antibody
Anti-Cck-4 antibody
Anti-Protein-Tyrosine Kinase 7 antibody
Anti-Pseudo Tyrosine Kinase Receptor 7 antibody
Anti-Tyrosine-Protein Kinase-Like 7 antibody
Anti-PTK7 antibody
Anti-CCK4 antibody

Information sourced from Uniprot.org

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