• Western blot analysis of extracts of various cell lines, using PTK7 antibody (STJ111881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using PTK7 antibody (STJ111881) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using PTK7 antibody (STJ111881) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human prostate using PTK7 antibody (STJ111881) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human prostate using PTK7 antibody (STJ111881) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using PTK7 antibody (STJ111881) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human stomach using PTK7 antibody (STJ111881) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using PTK7 antibody (STJ111881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PTK7 antibody (811-1070) (STJ111881)

SKU:
STJ111881

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PTK7 (811-1070) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTK7
Gene ID: 5754
Uniprot ID: PTK7_HUMAN
Immunogen Region: 811-1070
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 811-1070 of human PTK7 (NP_002812.2).
Immunogen Sequence: FLAKAQGLEEGVAETLVLVK SLQSKDEQQQLDFRRELEMF GKLNHANVVRLLGLCREAEP HYMVLEYVDLGDLKQFLRIS KSKDEKLKSQPLSTKQKVAL CTQVALGMEHLSNNRFVHKD LAARNCLVSAQRQVKVSALG LSKDVYNSEYYHFRQAWVPL RWMSPEAILEGDFSTKSDVW AFGVLMWEVFTHGEMPHGGQ ADDEVLADLQAGKARLPQPE GCPSKLYRLMQRCWALSPK
Tissue Specificity Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
Post Translational Modifications MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway.
Function Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
Protein Name Inactive Tyrosine-Protein Kinase 7
Colon Carcinoma Kinase 4
Cck-4
Protein-Tyrosine Kinase 7
Pseudo Tyrosine Kinase Receptor 7
Tyrosine-Protein Kinase-Like 7
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Colocalizes With Mmp14 At Cell Junctions
Also Localizes At The Leading Edge Of Migrating Cells
Alternative Antibody Names Anti-Inactive Tyrosine-Protein Kinase 7 antibody
Anti-Colon Carcinoma Kinase 4 antibody
Anti-Cck-4 antibody
Anti-Protein-Tyrosine Kinase 7 antibody
Anti-Pseudo Tyrosine Kinase Receptor 7 antibody
Anti-Tyrosine-Protein Kinase-Like 7 antibody
Anti-PTK7 antibody
Anti-CCK4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance