• Immunohistochemistry analysis of paraffin-embedded Rat leg bone using PTH1R Rabbit polyclonal antibody (STJ25209) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PTH1R antibody (STJ25209) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PTH1R antibody (27-593) (STJ25209)

SKU:
STJ25209

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PTH1R (27-593) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTH1R
Gene ID: 5745
Uniprot ID: PTH1R_HUMAN
Immunogen Region: 27-593
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-593 of human PTH1R (NP_000307.1).
Immunogen Sequence: DADDVMTKEEQIFLLHRAQA QCEKRLKEVLQRPASIMESD KGWTSASTSGKPRKDKASGK LYPESEEDKEAPTGSRYRGR PCLPEWDHILCWPLGAPGEV VAVPCPDYIYDFNHKGHAYR RCDRNGSWELVPGHNRTWAN YSECVKFLTNETREREVFDR LG
Tissue Specificity Expressed in most tissues. Most abundant in kidney, bone and liver.
Post Translational Modifications N-glycosylated.
Function Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
Protein Name Parathyroid Hormone/Parathyroid Hormone-Related Peptide Receptor
Pth/Pthrp Type I Receptor
Pth/Pthr Receptor
Parathyroid Hormone 1 Receptor
Pth1 Receptor
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-418555
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Parathyroid Hormone/Parathyroid Hormone-Related Peptide Receptor antibody
Anti-Pth/Pthrp Type I Receptor antibody
Anti-Pth/Pthr Receptor antibody
Anti-Parathyroid Hormone 1 Receptor antibody
Anti-Pth1 Receptor antibody
Anti-PTH1R antibody
Anti-PTHR antibody
Anti-PTHR1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance