• Western blot analysis of extracts of various cell lines, using Parathyroid Hormone (PTH) (PTH) rabbit monoclonal antibody (STJ11102529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Confocal imaging of paraffin-embedded Human thyroid tissue using Parathyroid Hormone (PTH) Rabbit monoclonal antibody (STJ11102529, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of extracts of Jurkat cells, using Parathyroid Hormone (PTH) (PTH) rabbit monoclonal antibody (STJ11102529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Confocal imaging of paraffin-embedded Mouse thyroid tissue using Parathyroid Hormone (PTH) Rabbit monoclonal antibody (STJ11102529, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of human thyroid using Parathyroid Hormone (PTH) (PTH) rabbit monoclonal antibody (STJ11102529) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Parathyroid Hormone (PTH) (PTH) Rabbit monoclonal antibody (STJ11102529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of mouse thyroid using Parathyroid Hormone (PTH) (PTH) rabbit monoclonal antibody (STJ11102529) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PTH antibody (1-100) [S9MR] (STJ11102529)

SKU:
STJ11102529

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Parathyroid Hormone (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTH
Gene ID: 5741
Uniprot ID: PTHY_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Parathyroid Hormone (PTH) (P01270).
Immunogen Sequence: MIPAKDMAKVMIVMLAICFL TKSDGKSVKKRSVSEIQLMH NLGKHLNSMERVEWLRKKLQ DVHNFVALGAPLAPRDAGSQ RPRKKEDNVLVESHEKSLGE
Function PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates 1-14C-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Protein Name Parathyroid Hormone
Pth
Parathormone
Parathyrin
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-418555
Cellular Localisation Secreted
Alternative Antibody Names Anti-Parathyroid Hormone antibody
Anti-Pth antibody
Anti-Parathormone antibody
Anti-Parathyrin antibody
Anti-PTH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance