• STJ70821 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70821 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70821 (8µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ70821 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

Anti-PTCH antibody (C-Term) (STJ70821)

SKU:
STJ70821-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PTCH (C-Term) is suitable for use in ELISA, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-8µg/ml
IF-Strong expression of the protein seen in the Golgi of HeLa and NIH3T3 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PTCH1
Gene ID: 5727
Uniprot ID: PTC1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_000255.1; NP_001077072.1; NP_001077071.1; NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1
Specificity: This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
Immunogen Sequence: ELQDVECEERPR
Post Translational Modifications Glycosylation is necessary for SHH binding. In the absence of Hh ligands, ubiquitination by ITCH at Lys-1426 promotes endocytosis and both proteasomal and lysosomal degradation.
Function Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
Protein Name Protein Patched Homolog 1
Ptc
Ptc1
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-5610787
Reactome: R-HSA-5632681
Reactome: R-HSA-5632684
Reactome: R-HSA-5635838
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Protein Patched Homolog 1 antibody
Anti-Ptc antibody
Anti-Ptc1 antibody
Anti-PTCH1 antibody
Anti-PTCH antibody

Information sourced from Uniprot.org

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