| Host: | Rabbit |
| Applications: | WB/IHC/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-PSMB8 (163-272) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PSMB8 |
| Gene ID: | 5696 |
| Uniprot ID: | PSB8_HUMAN |
| Immunogen Region: | 163-272 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 163-272 of human PSMB8 (NP_004150.1). |
| Immunogen Sequence: | LSMGSMICGWDKKGPGLYYV DEHGTRLSGNMFSTGSGNTY AYGVMDSGYRPNLSPEEAYD LGRRAIAYATHRDSYSGGVV NMYHMKEDGWVKVESTDVSD LLHQYREANQ |
| Post Translational Modifications | Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity. |
| Function | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes. |
| Protein Name | Proteasome Subunit Beta Type-8Low Molecular Mass Protein 7Macropain Subunit C13Multicatalytic Endopeptidase Complex Subunit C13Proteasome Component C13Proteasome Subunit Beta-5iReally Interesting New Gene 10 Protein |
| Database Links | Reactome: R-HSA-1169091Reactome: R-HSA-1234176Reactome: R-HSA-1236974Reactome: R-HSA-1236978Reactome: R-HSA-174084Reactome: R-HSA-174113Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-174184Reactome: R-HSA-180534Reactome: R-HSA-180585Reactome: R-HSA-187577Reactome: R-HSA-195253Reactome: R-HSA-202424Reactome: R-HSA-211733Reactome: R-HSA-2467813Reactome: R-HSA-2871837Reactome: R-HSA-349425Reactome: R-HSA-350562Reactome: R-HSA-382556Reactome: R-HSA-450408Reactome: R-HSA-4608870Reactome: R-HSA-4641257Reactome: R-HSA-4641258Reactome: R-HSA-5358346Reactome: R-HSA-5362768Reactome: R-HSA-5607761Reactome: R-HSA-5607764Reactome: R-HSA-5610780Reactome: R-HSA-5610783Reactome: R-HSA-5610785Reactome: R-HSA-5632684Reactome: R-HSA-5658442Reactome: R-HSA-5668541Reactome: R-HSA-5676590Reactome: R-HSA-5678895Reactome: R-HSA-5687128Reactome: R-HSA-5689603Reactome: R-HSA-5689880Reactome: R-HSA-68867Reactome: R-HSA-68949Reactome: R-HSA-69017Reactome: R-HSA-69481Reactome: R-HSA-69601Reactome: R-HSA-75815Reactome: R-HSA-8852276Reactome: R-HSA-8854050Reactome: R-HSA-8939236Reactome: R-HSA-8939902Reactome: R-HSA-8941858Reactome: R-HSA-8948751Reactome: R-HSA-8951664Reactome: R-HSA-9010553Reactome: R-HSA-9020702Reactome: R-HSA-909733Reactome: R-HSA-9604323Reactome: R-HSA-9755511Reactome: R-HSA-9762114Reactome: R-HSA-9824272Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Proteasome Subunit Beta Type-8 antibodyAnti-Low Molecular Mass Protein 7 antibodyAnti-Macropain Subunit C13 antibodyAnti-Multicatalytic Endopeptidase Complex Subunit C13 antibodyAnti-Proteasome Component C13 antibodyAnti-Proteasome Subunit Beta-5i antibodyAnti-Really Interesting New Gene 10 Protein antibodyAnti-PSMB8 antibodyAnti-LMP7 antibodyAnti-PSMB5i antibodyAnti-RING10 antibodyAnti-Y2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PSMB8 knockout (KO) A-549 cells, using [KO Validated] PSMB8 Rabbit polyclonal antibody (STJ11100809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89745/358219/STJ11100809_1__76245.1713122545.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PSMB8 knockout (KO) A-549 cells, using [KO Validated] PSMB8 Rabbit polyclonal antibody (STJ11100809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
