• STJ71044 (1µg/ml) staining of A431 (A) , HeLa (B) and HEK293 (C) nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71044 (1µg/ml) staining of NIH3T3 nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71044 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71044 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71044 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-PRPF31 antibody (Internal) (STJ71044)

SKU:
STJ71044-100

€390.63
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PRPF31 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 60kDa band observed in nuclear lysates of cell lines A431, HeLa, HEK293 and NIH3T3 (calculated MW of 55.4kDa according to Human NP_056444.3 and Mouse NP_081604.3). Recommended
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PRPF31
Gene ID: 26121
Uniprot ID: PRP31_HUMAN
Immunogen Region: Internal
Accession Number: NP_056444.3
Immunogen Sequence: KELGNSLDKCKNNEN
Post Translational Modifications Phosphorylated by PRP4K during spliceosome assembly.
Function Involved in pre-mRNA splicing as component of the spliceosome. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
Protein Name U4/U6 Small Nuclear Ribonucleoprotein Prp31
Pre-Mrna-Processing Factor 31
Serologically Defined Breast Cancer Antigen Ny-Br-99
U4/U6 Snrnp 61 Kda Protein
Protein 61k
Hprp31
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleus Speckle
Cajal Body
Predominantly Found In Speckles And In Cajal Bodies
Alternative Antibody Names Anti-U4/U6 Small Nuclear Ribonucleoprotein Prp31 antibody
Anti-Pre-Mrna-Processing Factor 31 antibody
Anti-Serologically Defined Breast Cancer Antigen Ny-Br-99 antibody
Anti-U4/U6 Snrnp 61 Kda Protein antibody
Anti-Protein 61k antibody
Anti-Hprp31 antibody
Anti-PRPF31 antibody
Anti-PRP31 antibody

Information sourced from Uniprot.org

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