• Confocal imaging of paraffin-embedded Human spleen using Proteinase 3/PR3 Rabbit monoclonal antibody (STJ11103110, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of lysates from Mouse liver, using Proteinase 3/PR3 Rabbit monoclonal antibody (STJ11103110) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Proteinase 3/PR3 antibody (1-100) [S0MR] (STJ11103110)

SKU:
STJ11103110

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Proteinase 3/PR3 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRTN3
Gene ID: 5657
Uniprot ID: PRTN3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Proteinase 3/PR3 (P24158).
Immunogen Sequence: MAHRPPSPALASVLLALLLS GAARAAEIVGGHEAQPHSRP YMASLQMRGNPGSHFCGGTL IHPSFVLTAAHCLRDIPQRL VNVVLGAHNVRTQEPTQQHF
Tissue Specificity Expressed in polymorphonuclear leukocytes (at protein level). Expressed in neutrophils (at protein level). Expressed in differentiating neutrophils.
Function Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.
Protein Name Myeloblastin
Agp7
C-Anca Antigen
Leukocyte Proteinase 3
Pr-3
Pr3
Neutrophil Proteinase 4
Np-4
P29
Wegener Autoantigen
Database Links Reactome: R-HSA-140875
Reactome: R-HSA-449836
Reactome: R-HSA-6798695
Reactome: R-HSA-6803157
Cellular Localisation Cytoplasmic Granule
Secreted
Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Membrane Raft
Localizes Predominantly To Azurophil Granules (Primary Secretory Granules) In Neutrophils
Secreted Upon Neutrophil Stimulation By Tnf-Alpha
Lipopolysaccharide (Lps)
Fmlp And Cxcl8/Il8 Or During Neutrophil Transmigration
Following Secretion Tethered To The Cell Membrane By Cd177
Alternative Antibody Names Anti-Myeloblastin antibody
Anti-Agp7 antibody
Anti-C-Anca Antigen antibody
Anti-Leukocyte Proteinase 3 antibody
Anti-Pr-3 antibody
Anti-Pr3 antibody
Anti-Neutrophil Proteinase 4 antibody
Anti-Np-4 antibody
Anti-P29 antibody
Anti-Wegener Autoantigen antibody
Anti-PRTN3 antibody
Anti-MBN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance