Anti-Proliferation Marker antibody [IPO-38] (STJ16101069)

Anti-Proliferation Marker antibody [IPO-38] (STJ16101069)

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STJ16101069
€414.38 - €1,845.63
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Host: Mouse
Applications: ELISA/WB/IP/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Mouse monoclonal anti-Proliferation Marker for use in ELISA, WB, IP and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
Clonality : Monoclonal
Clone ID : IPO-38
Conjugation: Unconjugated
Isotype: IgMk
Formulation: PBS with 0.02% Sodium Azide.
Purification: Affinity purified from tissue culture.
Concentration: Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
Dilution Range: ELISA (solid phase: 0, 1-100 µg/ml; tracer: 0, 001-100 µg/ml for 30 min at RT). Immunoblotting (1-2 µg/ml). Immunoprecipitation (1-2 µg per 100-500 µg of total cell lysate protein/1 ml of anti-mouse coated Sepharose-4B suspension). Immunohistology (1
Storage Instruction: Store for up to 1 year at 2-8°C upon receipt.
Gene Symbol: MKI67
Gene ID: 4288
Uniprot ID: KI67_HUMAN
Immunogen: A BALB/c mouse was immunized with spleen cells of a patient with hairy cell leukemia. Fusion partner: SP2/0.
Post Translational Modifications Hyperphosphorylated by CDK1 in mitosis.hyperphosphorylatiom prevents undergoing liquid-liquid phase separation. Dephosphorylated by PPP1CC at the onset of anaphase. Dephosphorylated by protein phosphatase 2A (PP2A) at the onset of anaphase. Dephosphorylation by protein phosphatase 2A (PP2A) and simultaneous exposure of the positively charged patch (CP) during mitotic exit induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface. Ubiquitinated by the APC/C complex after neuronal progenitors exit mitosis during brain development, leading to clearance from constitutive heterochromatin.
Function Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis. Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface. The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space. Mechanistically, dephosphorylation during mitotic exit and simultaneous exposure of a conserved basic patch induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface, promoting coalescence of neighboring chromosome surfaces and clustering of chromosomes. Binds premature ribosomal RNAs during anaphase.promoting liquid-liquid phase separation. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization.it is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in mitotic chromosome.
Protein Name Proliferation Marker Protein Ki-67
Antigen Identified By Monoclonal Antibody Ki-67
Antigen Ki-67
Antigen Ki67
Database Links
Cellular Localisation Chromosome
Nucleus
Nucleolus
During Early Mitosis
Relocalizes From Nucleoli To The Surface Of The Mitotic Chromosome
The Perichromosomal Layer
And Covers A Substantial Fraction Of The Mitotic Chromosome Surface
Associates With Satellite Dna In G1 Phase
Binds Tightly To Chromatin In Interphase
Chromatin-Binding Decreases In Mitosis When It Associates With The Surface Of The Condensed Chromosomes
Predominantly Localized In The G1 Phase In The Perinucleolar Region
In The Later Phases It Is Also Detected Throughout The Nuclear Interior
Being Predominantly Localized In The Nuclear Matrix
Alternative Antibody Names Anti-Proliferation Marker Protein Ki-67 antibody
Anti-Antigen Identified By Monoclonal Antibody Ki-67 antibody
Anti-Antigen Ki-67 antibody
Anti-Antigen Ki67 antibody
Anti-MKI67 antibody

Information sourced from Uniprot.org

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