| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PRDX4 (10-110) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRDX4 |
| Gene ID: |
10549 |
| Uniprot ID: |
PRDX4_HUMAN |
| Immunogen Region: |
10-110 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 10-110 of human Peroxiredoxin 4 (PRDX4) (NP_006397.1). |
| Immunogen Sequence: |
TTPDHGRHRRLLLLPLLLFL LPAGAVQGWETEERPRTREE ECHFYAGGQVYPGEASRVSV ADHSLHLSKAKISKPAPYWE GTAVIDGEFKELKLTDYRGK Y |
| Post Translational Modifications | The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond. |
| Function | Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. |
| Protein Name | Peroxiredoxin-4Antioxidant Enzyme Aoe372Aoe37-2Peroxiredoxin IvPrx-IvThioredoxin Peroxidase Ao372Thioredoxin-Dependent Peroxide Reductase A0372Thioredoxin-Dependent Peroxiredoxin 4 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | CytoplasmEndoplasmic ReticulumCotranslationally Translocated To And Retained Within The Endoplasmic ReticulumA Small Fraction Of The Protein Is Cytoplasmic |
| Alternative Antibody Names | Anti-Peroxiredoxin-4 antibodyAnti-Antioxidant Enzyme Aoe372 antibodyAnti-Aoe37-2 antibodyAnti-Peroxiredoxin Iv antibodyAnti-Prx-Iv antibodyAnti-Thioredoxin Peroxidase Ao372 antibodyAnti-Thioredoxin-Dependent Peroxide Reductase A0372 antibodyAnti-Thioredoxin-Dependent Peroxiredoxin 4 antibodyAnti-PRDX4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and NME1/NM23A knockout (KO) 293T cells, using [KO Validated] NME1/NM23A Rabbit polyclonal antibody (STJ119972) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98757/382809/STJ119972_1__49721.1713151303.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NME1/NM23A knockout (KO) 293T cells, using [KO Validated] NME1/NM23A Rabbit polyclonal antibody (STJ119972) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")