• Western blot analysis of extracts of various cell lines, using (STJ11105745) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PPP6C antibody (206-305) [S5745RM] (STJ11105745)

SKU:
STJ11105745

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Serine/Threonine-Protein Phosphatase 6 Catalytic Subunit (206-305) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5745RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP6C
Gene ID: 5537
Uniprot ID: PPP6_HUMAN
Immunogen Region: 206-305
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 206-305 of human PPP6C (O00743).
Immunogen Sequence: AISPRGAGWLFGAKVTNEFV HINNLKLICRAHQLVHEGYK FMFDEKLVTVWSAPNYCYRC GNIASIMVFKDVNTREPKLF RAVPDSERVIPPRTTTPYFL
Tissue Specificity Ubiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen.
Function Catalytic subunit of protein phosphatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. During mitosis, regulates spindle positioning. Down-regulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7. Participates also in the innate immune defense against viruses by desphosphorylating RIGI, an essential step that triggers RIGI-mediated signaling activation. Also regulates innate immunity by acting as a negative regulator of the cGAS-STING pathway: mediates dephosphorylation and inactivation of CGAS and STING1. CGAS dephosphorylation at 'Ser-435' impairs its ability to bind GTP, thereby inactivating it.
Protein Name Serine/Threonine-Protein Phosphatase 6 Catalytic Subunit
Pp6c Cleaved Into - Serine/Threonine-Protein Phosphatase 6 Catalytic Subunit - N-Terminally Processed
Database Links Reactome: R-HSA-171319
Reactome: R-HSA-204005
Cellular Localisation Mitochondrion
Cytoplasm
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 6 Catalytic Subunit antibody
Anti-Pp6c Cleaved Into - Serine/Threonine-Protein Phosphatase 6 Catalytic Subunit - N-Terminally Processed antibody
Anti-PPP6C antibody
Anti-PPP6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance