| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Calcineurin A (422-521) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S8MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PPP3CA |
| Gene ID: |
5530 |
| Uniprot ID: |
PP2BA_HUMAN |
| Immunogen Region: |
422-521 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 422-521 of human Calcineurin A (Q08209). |
| Immunogen Sequence: |
TLKGLTPTGMLPSGVLSGGK QTLQSATVEAIEADEAIKGF SPQHKITSFEEAKGLDRINE RMPPRRDAMPSDANLNSINK ALTSETNGTDSNGSNSSNIQ |
| Tissue Specificity | Expressed in keratinocytes (at protein level). Expressed in lymphoblasts (at protein level). |
| Function | Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Many of the substrates contain a PxIxIT motif and/or a LxVP motif. In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation. In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion. Positively regulates the CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals. Dephosphorylates heat shock protein HSPB1. Dephosphorylates and activates transcription factor NFATC1. In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32. May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins. Dephosphorylates transcription factor TFEB at 'Ser-211' following Coxsackievirus B3 infection, promoting nuclear translocation. Required for postnatal development of the nephrogenic zone and superficial glomeruli in the kidneys, cell cycle homeostasis in the nephrogenic zone, and ultimately normal kidney function. Plays a role in intracellular AQP2 processing and localization to the apical membrane in the kidney, may thereby be required for efficient kidney filtration. Required for secretion of salivary enzymes amylase, peroxidase, lysozyme and sialic acid via formation of secretory vesicles in the submandibular glands. Required for calcineurin activity and homosynaptic depotentiation in the hippocampus. Required for normal differentiation and survival of keratinocytes and therefore required for epidermis superstructure formation. Positively regulates osteoblastic bone formation, via promotion of osteoblast differentiation. Positively regulates osteoclast differentiation, potentially via NFATC1 signaling. May play a role in skeletal muscle fiber type specification, potentially via NFATC1 signaling. Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB. Required for antigen-specific T-cell proliferation response. Dephosphorylates KLHL3, promoting the interaction between KLHL3 and WNK4 and subsequent degradation of WNK4. Negatively regulates SLC9A1 activity. |
| Protein Name | Protein Phosphatase 3 Catalytic Subunit AlphaCam-Prp Catalytic SubunitCalcineurin A AlphaCalmodulin-Dependent Calcineurin A Subunit Alpha IsoformCna AlphaSerine/Threonine-Protein Phosphatase 2b Catalytic Subunit Alpha Isoform |
| Database Links | Reactome: R-HSA-180024Reactome: R-HSA-2025928Reactome: R-HSA-2871809Reactome: R-HSA-4086398Reactome: R-HSA-5607763 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinSarcolemmaMyofibrilSarcomereZ LineCell ProjectionDendritic SpineColocalizes With Actn1 And Myoz2 At The Z Line In Heart And Skeletal MuscleRecruited To The Cell Membrane By Scaffold Protein Akap5 Following L-Type Ca(2+)-Channel Activation |
| Alternative Antibody Names | Anti-Protein Phosphatase 3 Catalytic Subunit Alpha antibodyAnti-Cam-Prp Catalytic Subunit antibodyAnti-Calcineurin A Alpha antibodyAnti-Calmodulin-Dependent Calcineurin A Subunit Alpha Isoform antibodyAnti-Cna Alpha antibodyAnti-Serine/Threonine-Protein Phosphatase 2b Catalytic Subunit Alpha Isoform antibodyAnti-PPP3CA antibodyAnti-CALNA antibodyAnti-CNA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
