• Immunofluorescence analysis of U-2 OS cells using PPP2CB antibody (STJ25086) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using PPP2CB antibody (STJ25086) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat kidney using PPP2CB antibody (STJ25086) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using PPP2CB antibody (STJ25086) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using PPP2CB antibody (STJ25086) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Anti-PPP2CB antibody (STJ25086)


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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-PPP2CB antibody is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:100-1:200
IF: 1:50-1:200
Gene ID: 5516
Gene Symbol: PPP2CB
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PPP2CB (NP_001009552.1).
Post Translational Modifications Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization. Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. May be monoubiquitinated by NOSIP.
Function PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
Protein Name Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Beta Isoform
Database Links Reactome: R-HSA-113501
Reactome: R-HSA-1295596
Reactome: R-HSA-141444
Reactome: R-HSA-163685
Reactome: R-HSA-163767
Reactome: R-HSA-180024
Reactome: R-HSA-195253
Reactome: R-HSA-196299
Reactome: R-HSA-198753
Reactome: R-HSA-202670
Reactome: R-HSA-2465910
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-389513
Reactome: R-HSA-432142
Reactome: R-HSA-4641262
Reactome: R-HSA-5339716
Reactome: R-HSA-5358747
Reactome: R-HSA-5358749
Reactome: R-HSA-5358751
Reactome: R-HSA-5358752
Reactome: R-HSA-5467337
Reactome: R-HSA-5467340
Reactome: R-HSA-5467348
Reactome: R-HSA-5663220
Reactome: R-HSA-5673000
Reactome: R-HSA-5675221
Reactome: R-HSA-6804757
Reactome: R-HSA-6811558
Reactome: R-HSA-68877
Reactome: R-HSA-69231
Reactome: R-HSA-69273
Reactome: R-HSA-9634600
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Spindle Pole
In Prometaphase Cells
But Not In Anaphase Cells
Localizes At Centromeres
During Mitosis
Also Found At Spindle Poles
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Beta Isoform antibody
Anti-Pp2a-Beta antibody
Anti-PPP2CB antibody

Information sourced from Uniprot.org

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