• Western blot analysis of various lysates, using PP2A Catalytic Alpha antibody (STJ28785) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of HeLa cells using PP2A Catalytic Alpha antibody (STJ28785) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PP2A Catalytic Alpha antibody (STJ28785) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g PP2A Catalytic Alpha antibody (STJ28785). Western blot was performed from the immunoprecipitate using PP2A Catalytic Alpha antibody (STJ28785) at a dilution of 1:1000.
  • Immunoprecipitation analysis of 25 Mu g extracts of Rat brain cells using 3 Mu g PP2A Catalytic Alpha antibody (STJ28785). Western blot was performed from the immunoprecipitate using PP2A Catalytic Alpha antibody (STJ28785) at a dilution of 1:1000.

Anti-PPP2CA antibody (1-309) (STJ28785)

SKU:
STJ28785

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP2CA (1-309) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP2CA
Gene ID: 5515
Uniprot ID: PP2AA_HUMAN
Immunogen Region: 1-309
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PP2A Catalytic Alpha (NP_002706.1).
Immunogen Sequence: MDEKVFTKELDQWIEQLNEC KQLSESQVKSLCEKAKEILT KESNVQEVRCPVTVCGDVHG QFHDLMELFRIGGKSPDTNY LFMGDYVDRGYYSVETVTLL VALKVRYRERITILRGNHES RQITQVYGFYDECLRKYGNA NVWKYFTDLFDYLPLTALVD GQIFCLHGGLSPSIDTLDHI RALDRLQEVPHEGPMCDLLW SDPDDRGGWGISPRGAGYTF GQDISETFNHANGLTLVSR
Post Translational Modifications Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization. Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. Polyubiquitinated, leading to its degradation by the proteasome.
Function PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint. Mediates dephosphorylation of MYC.promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC. Mediates dephosphorylation of FOXO3.promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3. Catalyzes dephosphorylation of the pyrin domain of NLRP3, promoting assembly of the NLRP3 inflammasome.
Protein Name Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform
Pp2a-Alpha
Replication Protein C
Rp-C
Database Links Reactome: R-HSA-113501
Reactome: R-HSA-1295596
Reactome: R-HSA-141444
Reactome: R-HSA-163685
Reactome: R-HSA-163767
Reactome: R-HSA-180024
Reactome: R-HSA-195253
Reactome: R-HSA-196299
Reactome: R-HSA-198753
Reactome: R-HSA-202670
Reactome: R-HSA-2465910
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-2995383
Reactome: R-HSA-389513
Reactome: R-HSA-432142
Reactome: R-HSA-4641262
Reactome: R-HSA-5339716
Reactome: R-HSA-5358747
Reactome: R-HSA-5358749
Reactome: R-HSA-5358751
Reactome: R-HSA-5358752
Reactome: R-HSA-5467337
Reactome: R-HSA-5467340
Reactome: R-HSA-5467348
Reactome: R-HSA-5663220
Reactome: R-HSA-5673000
Reactome: R-HSA-5675221
Reactome: R-HSA-6804757
Reactome: R-HSA-6811558
Reactome: R-HSA-68877
Reactome: R-HSA-69231
Reactome: R-HSA-69273
Reactome: R-HSA-9634600
Reactome: R-HSA-9648025
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Chromosome
Centromere
Cytoskeleton
Spindle Pole
In Prometaphase Cells
But Not In Anaphase Cells
Localizes At Centromeres
During Mitosis
Also Found At Spindle Poles
Centromeric Localization Requires The Presence Of Sgo2
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform antibody
Anti-Pp2a-Alpha antibody
Anti-Replication Protein C antibody
Anti-Rp-C antibody
Anti-PPP2CA antibody

Information sourced from Uniprot.org

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