• Immunofluorescence analysis of L929 cells using PPP1R9B Rabbit polyclonal antibody (STJ119355) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PPP1R9B Rabbit polyclonal antibody (STJ119355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PPP1R9B antibody (250-350) (STJ119355)

SKU:
STJ119355

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP1R9B (250-350) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1R9B
Gene ID: 84687
Uniprot ID: NEB2_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human PPP1R9B (NP_115984.3).
Immunogen Sequence: VFQPPPPPPPAPSGDAPAEK ERCPAGQQPPQHRVAPARPP PKPREVRKIKPVEVEESGES EAESAPGEVIQAEVTVHAAL ENGSTVATAASPAPEEPKAQ A
Post Translational Modifications Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 is mediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association with F-actin, but does not affect its binding to PP1.
Function Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1. Required for hepatocyte growth factor (HGF)-induced cell migration.
Protein Name Neurabin-2
Neurabin-Ii
Protein Phosphatase 1 Regulatory Subunit 9b
Spinophilin
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Cell Projection
Dendritic Spine
Postsynaptic Density
Synapse
Cell Junction
Adherens Junction
Cell Membrane
Lamellipodium
Filopodium
Ruffle Membrane
Enriched At Synapse And Cadherin-Based Cell-Cell Adhesion Sites
In Neurons
Both Cytosolic And Membrane-Associated
And Highly Enriched In The Postsynaptic Density Apposed To Exitatory Synapses
Colocalizes With Ppp1r2 At Actin-Rich Adherens Junctions In Epithelial Cells And In Dendritic Spines
Accumulates In The Lamellipodium
Filopodium And Ruffle Membrane In Response To Hepatocyte Growth Factor (Hgf) Treatment
Alternative Antibody Names Anti-Neurabin-2 antibody
Anti-Neurabin-Ii antibody
Anti-Protein Phosphatase 1 Regulatory Subunit 9b antibody
Anti-Spinophilin antibody
Anti-PPP1R9B antibody
Anti-PPP1R6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance