Anti-PPARA antibody (6-55 aa) (STJ95200)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human PPAR-alpha at the amino acid range 6-55
General Information
| Short Description | Rabbit polyclonal anti-Peroxisome proliferator-activated receptor alpha (6-55 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:10000-20000IHC 1:50-300IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PPARA |
| Gene ID | 5465 |
| Uniprot ID | PPARA_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human PPAR-alpha at the amino acid range 6-55 |
| Immunogen Region | 6-55 aa |
| Specificity | PPAR-Alpha Polyclonal Antibody detects endogenous levels of PPAR-Alpha protein. |
Additional Info
| Post Translational Modifications | Ubiquitinated by E3 ubiquitin-protein ligase HUWE1.leading to proteasomal degradation. Phosphorylated. |
| Function | Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2. |
| Protein Name | Peroxisome Proliferator-Activated Receptor AlphaPpar-AlphaNuclear Receptor Subfamily 1 Group C Member 1 |
| Database Links | Reactome: R-HSA-1368082Reactome: R-HSA-1368108Reactome: R-HSA-1989781Reactome: R-HSA-2151201Reactome: R-HSA-2426168Reactome: R-HSA-381340Reactome: R-HSA-383280Reactome: R-HSA-400206Reactome: R-HSA-400253Reactome: R-HSA-4090294Reactome: R-HSA-9707564Reactome: R-HSA-9707616Reactome: R-HSA-9844594 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Peroxisome Proliferator-Activated Receptor Alpha antibodyAnti-Ppar-Alpha antibodyAnti-Nuclear Receptor Subfamily 1 Group C Member 1 antibodyAnti-PPARA antibodyAnti-NR1C1 antibodyAnti-PPAR antibody |
Information sourced from Uniprot.org