| Host: | Goat |
| Applications: | Pep-ELISA/WB/IF/FC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-POU3F3/BRN1/OCT8 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 48kDa band observed in Mouse Spinal Cord lysates and approx. 50kDa in Mouse Brain lysates (calculated MW of 50.3kDa according to Human NP_006227.1 and 50.2kDa according to Mouse |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | POU3F3 |
| Gene ID: | 5455 |
| Uniprot ID: | PO3F3_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_006227.1 |
| Immunogen Sequence: | HMLSHAHQWVTAL |
| Function | Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction.the enhancer element contains the octamer motif (5'-ATTTGCAT-3'). |
| Protein Name | Pou Domain - Class 3 - Transcription Factor 3Brain-Specific Homeobox/Pou Domain Protein 1Brain-1Brn-1Octamer-Binding Protein 8Oct-8Octamer-Binding Transcription Factor 8Otf-8 |
| Database Links | Reactome: R-HSA-9831926 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Pou Domain - Class 3 - Transcription Factor 3 antibodyAnti-Brain-Specific Homeobox/Pou Domain Protein 1 antibodyAnti-Brain-1 antibodyAnti-Brn-1 antibodyAnti-Octamer-Binding Protein 8 antibodyAnti-Oct-8 antibodyAnti-Octamer-Binding Transcription Factor 8 antibodyAnti-Otf-8 antibodyAnti-POU3F3 antibodyAnti-BRN1 antibodyAnti-OTF8 antibody |
Information sourced from Uniprot.org