• Immunofluorescence analysis of MCF7 cells using POLG2 antibody (STJ28778). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using POLG2 antibody (STJ28778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using POLG2 antibody (STJ28778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using POLG2 antibody (STJ28778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using POLG2 antibody (STJ28778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using POLG2 antibody (STJ28778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLG2 antibody (216-485) (STJ28778)

SKU:
STJ28778

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLG2 (216-485) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLG2
Gene ID: 11232
Uniprot ID: DPOG2_HUMAN
Immunogen Region: 216-485
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 216-485 of human POLG2 (NP_009146.2).
Immunogen Sequence: HPVFDTKQIRNGVKSIGEKT EASLVWFTPPRTSNQWLDFW LRHRLQWWRKFAMSPSNFSS SDCQDEEGRKGNKLYYNFPW GKELIETLWNLGDHELLHMY PGNVSKLHGRDGRKNVVPCV LSVNGDLDRGMLAYLYDSFQ LTENSFTRKKNLHRKVLKLH PCLAPIKVALDVGRGPTLEL RQVCQGLFNELLENGISVWP GYLETMQSSLEQLYSKYDEM SILFTVLVTETTLENGLIH
Function Mitochondrial polymerase processivity subunit. It regulates the polymerase and exonuclease activities promoting processive DNA synthesis. Binds to ss-DNA.
Protein Name Dna Polymerase Subunit Gamma-2 - Mitochondrial
Dna Polymerase Gamma Accessory 55 Kda Subunit
P55
Mitochondrial Dna Polymerase Accessory Subunit
Mtpolb
Polg-Beta
Database Links Reactome: R-HSA-2151201
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Dna Polymerase Subunit Gamma-2 - Mitochondrial antibody
Anti-Dna Polymerase Gamma Accessory 55 Kda Subunit antibody
Anti-P55 antibody
Anti-Mitochondrial Dna Polymerase Accessory Subunit antibody
Anti-Mtpolb antibody
Anti-Polg-Beta antibody
Anti-POLG2 antibody
Anti-MTPOLB antibody

Information sourced from Uniprot.org

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